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Topic: Acid Hydrolysis Fat, Fat-SBR qstn  (Read 2793 times)

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Offline ken3009

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Acid Hydrolysis Fat, Fat-SBR qstn
« on: October 10, 2012, 03:22:24 AM »
i have used this method to measure fats, but sometimes for samples like honey i do not wash the fat flasks after taking the first constant weight because i dont see any black or insoluble particles in the flask. but the result is too high (like 4.5g/100g, and it should be 0.5g/100g for example). is it possible there could be sugars present in the flask? i extracted carefully with the mojonnier tubes, but could sugars dissolve in the ether?? how do i identify if a flask needs washing or not if there are no black/brown particles in it???

Offline Hunter2

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Re: Acid Hydrolysis Fat, Fat-SBR qstn
« Reply #1 on: October 10, 2012, 05:05:14 AM »
Did you compare with a new flask?  Sugar should be soluble in water.

Offline Arkcon

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Re: Acid Hydrolysis Fat, Fat-SBR qstn
« Reply #2 on: October 10, 2012, 11:59:00 AM »
Briefly, if the method calls for a washing, you should do it.  If the method doesn't say that you shouldn't wash a flask, you should wash thoroughly and dry to constant weight before performing any analytical procedure.  That's just good QA laboratory hygiene.
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Offline ken3009

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Re: Acid Hydrolysis Fat, Fat-SBR qstn
« Reply #3 on: October 12, 2012, 01:37:05 AM »
no what im sayingis, how do i know which samples to wash and which not to. i know for sure that flasks wit black/brown insoluble residue i need to wash, but can the residue be almost the color of fat, or whitish??

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