I hope everyone to have a nice New Year's Eve tonight!
However, before night comes, would you be so kind as to answer me?
I know detection of disulphide linkages (when we determine the primary structure of a protein) can be carried out with the help of mass spectrometry (MALDI spectra).
I read of a different approach based on a so-called diagonal cellulose acetate electroforesis. I just know you oxidize, with performic acid, SH-groups (only those which formed disulphide lingages) to SO4--, and then you perform your electroforesis.
The questions are:
- what does 'diagonal' mean in this situation?
- how do SO4-- groups 'behave' or how can you recognize them?
Thank you and have nice parties! :partytime2: