Hello everyone, I was looking around for some help online with a lab report I have and your website looks very helpful, so I decided to see if anyone here would be willing to give me a hand with some work I have. To give some background, I am a Psychology student specialising in Neuroscience and am currently studying a Biochemistry course in Molecular Neuroscience in addition to my more Cognitive Neuroscience courses in the Psychology department. For this reason I have not covered many of the basics of biochemical reactions, so could do with some help with a few calculations.
I have completed the lab work and am just trying to calculate various things with my data set right now. The main thing I am stuck on is measuring total AChE activity in some samples. I have the equation:
Total activity=ΔA/ε*l*3*total volume/0.1*dilution factor*10^3
I believe change in absorbance is just the change in absorbance over 1 minute, but the lab book gives no details about ε*l and I do not believe it was mentioned in the lab. Not knowing this factor is the main point I am stuck on as I think I know what the other terms mean. I have seen that it refers to the extinction coefficient, but do not know how to work this out (or if ε on its own refers to this, what l is).
Any help or comments would be greatly appreciated! Also, if anyone could recommend a textbook, website or any other resource that has details of how to calculate and graph enzyme and protein assays that would be extremely helpful. I have looked in many of the biochemistry books in the library, but most of them seem to cover the theory more than the actual calculations.