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Topic: GC Analysis Lab  (Read 2348 times)

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Offline ahhppull

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GC Analysis Lab
« on: November 25, 2012, 11:02:59 AM »
How do I find the concentration and response factor of each compound using the retention time and the area? 

My data is
Retention Time                   Peak Area
1.850                                  4489.8740
3.266                                  8536.9520
4.933                                 10534.9770

Offline curiouscat

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Re: GC Analysis Lab
« Reply #1 on: November 25, 2012, 11:06:10 AM »
All you can do with this meagre data is

% conc. = area / [ sum of areas ]

But won't be very accurate at all.

Offline ahhppull

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Re: GC Analysis Lab
« Reply #2 on: November 25, 2012, 11:09:34 AM »
Also for this standard, I got the percentage of each compound.  Would this help?  Octace (1.15%), Nonane(1.91%) and Decane (2.24%)

Offline sschoe2

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Re: GC Analysis Lab
« Reply #3 on: February 16, 2013, 08:34:48 PM »
From the data you have the best you can do is a percent report which as best is an estimate for most detectors. For the FID similar compounds often have similar response factors. For MSD and others not really. Also keep in mind you can only see volatile compounds.

For an accurate quantitation you need a reference compound and calibration curve. Without a method development the only good way to do it would be standard addition. Otherwise you have no idea what effect the matrix has ie the recovery.

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