phosphate buffered saline
Phosphate buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research. It is a water-based salt solution containing sodium chloride, sodium phosphate, and, in some formulations, potassium chloride and potassium phosphate. The buffer's phosphate groups help to maintain a constant pH.
At Beginning I made a 0.5 Molar stock solution (250 miligram sodium butyrate + 4.545 cc phosphate buffered saline(PBS)). After that did not dissolve , again I made a 0.1 molar stock solution (Previous solutions + 18.160 cc phosphate buffered saline(PBS)) But it was not effective.
Thanks for your time.