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Topic: fluorescence detection  (Read 1817 times)

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Offline zenith

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fluorescence detection
« on: January 21, 2013, 12:35:55 AM »
Am using nile red for my experiments without any solvents. And am supposed to take the fluorescence imaging. As far as i know nile red fluoresce only when it reacts with organic solvents or lipids? Anyone with any idea or alternative for fluorescence ?

Offline Arkcon

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Re: fluorescence detection
« Reply #1 on: January 21, 2013, 10:07:46 AM »
You can certainly try to make a fluorescence image without solvent or lipid.  It would all depend on the efficiency of you image system and what your application is.  Have you tried it?  What did you get?
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

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