April 25, 2024, 08:10:04 PM
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Topic: determination of moles of unknown gas from calibration curve and peak height  (Read 1778 times)

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Offline csinha

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I am working on a lab and I am unsure how to get the concentration of three gases in an unknown sample. I was required to create a calibration curve for peak area vs volume of analyte injected. The analyte gas is composed of methyl ethyl ketone, toluene and 2-butanol. The unknown sample contains these same gases. I determined the concentration of moles of each  in the calibration solution by pV=nRT where V is the volume of analyte injected into the GC (50 ul). I do not know how to find the molar concentration of the gases in the unknown sample. I know that peak area is proportional to concentration of an analyte but I do not know how to determine it with the information I have.

Offline Borek

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If I understand correctly what the problem is - use the calibration curve to determine apparent volume of the sample injected.
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