[SiberiaFrogUnit]

Hello,

Today I got a question about determination of nitrate in water.
I know that Nitrate in water can be analyzed by spectrophotometer or Ion chromatography.
But He who gave me a question said,
there is a method that using Micellar liquid chromatography.

So I searched and found a paper,

Title:
Determination of lnorganic Anions by High-performance Liquid
Chromatography Using a Micellar Mobile Phase

Author:
Frank G. P. Mullins and (the late) Gordon F. Kirkbright
ANALYST, SEPTEMBER 1984, VOL. 109

that paper says, mobile phase that contains Hexadecyltrimethylammonium chloride
can separate Nitrate with No pretreatment to water.

Could it possible?

I don't know about that well but..
If that paper written in 1984 is so good,
why that paper's method cannot up to Standard method book??

Anybody knows about this,
please tell me quick..

best regard!

[Arkcon]

OK, I don't have credentials to look up more than the abstracts for papers on this topic.  What I read is that with a hydrophobic column, you can separate ions with this particular detergent as a mobile phase.  This seems entirely plausible.  Here's a question for you -- why do you want to do it this way?  We'll need to review the actual paper, but there's nothing in the abstract to tell us how detection of the anions is done.  You'll really have to be sure your sample is free of other things if we're going to use very short wavelength UV detection.  Whereas using an IC, with suppressor and conductivity detector is a well known application.

[SiberiaFrogUnit]

thanks Arkcon, this is the answer for your question,

the reason why I want to use this method is simple.
our lab has HPLC but doesn't have spectrophotometer and IC.
and it's just one off request from another department so I think we are not going to buy one of them.

and if you don't mind could you let me know your e-mail address?
I bought this paper, so I don't know whether I upload pdf file on this board.
If I can, I will upload here or not I will send you by e-mail.

Thank you

[Arkcon]

If the reference you have is meant to be shared by all, then you can attach it to you next posting.  Just cloick on Additional Option ... below:

[SiberiaFrogUnit]

ok, this is the paper~

[ken3009]

i test nitrate in water using BP method. maybe u need to write about that? maybe its USP for u.
blank is 4.5ml epure and 0.5mo 2ppm nitrate standard solution

[sweetyee]

I want to determine the total concentration of N in a liquid fertilizer. As for total N, which forms of N should i be doing? Is it determining ammonia, ammonium, nitrite, nitrate, TKN or TAN? Pls advise.