So I'm working on a project where I need to determine the total ATP that a fatty acid would produce. I've done similar projects, but none were quite to this extent, and they were all single fatty acids. The one I'm working on now is a triacyl glycerol.
This is it: http://oi39.tinypic.com/4ftiqp.jpg
I believe a lipase would release the 3 fatty acids and I'd also end up with glycerol. So I ignored glycerol for right now and looked at the fatty acids individually, and this is what I came up with:
Each chain requires 2 ATP to enter the mitochondria.
FA1 and FA2 yield 4 Acetyl-CoA in 3 turns of beta-oxidation. FA3 yields 3 Acetyl-CoA and 1 propionyl-CoA in 3 turns of beta-oxidation.
Since 5 ATP are generated per turn, all 3 FAs generate 15 ATP each.
Propionyl-CoA goes to form succinyl-CoA, costing 1 ATP.
In the TCA cycle, 12 ATP (1 ATP directly, and 1 FADH2 = 2 ATPs, 3 NADH = 9 ATPs) are generated per Acetyl-CoA, giving 48 ATP for FA1 and FA2, and 36 for FA3.
Succinyl-CoA enters the TCA cycle and goes to form succinate, then fumerate, then malate, and finally oxaloacetate, generating a total of 5 ATP (again saying 1 GTP = 1 ATP, 1 FADH2 = 2 ATP, and 1 NADH = 3 ATP).
For all of this combined, I have a total of 175 ATP, and that's where I stopped.
For the glycerol, I was going to convert it to DHAP and send it through glycolysis. But before that, I wanted to go back through and see if I messed something up, and maybe get a second opinion?