[roygitanjali]

Standard: 10 mg of std. sample dissolved in 100 ml MeOH, 2 ml from this 100 ml is diluted to 50 ml MeOH.

Sample: To 100 gm of the sample add 50 ml dimethyl sulphoxide, shake & keep at 40'C for 20 mins. Cool & add 50 ml of 50% MeOH. Add 100 ml n-hexane and pass through anhydrous sodium sulphate. Pipette 75 ml of this n-hexane layer and evaporate to dryness in a round bottom flask. Dissolve the residue left after evaporation in 5 ml MeOH.

[Arkcon]

Per forum rules you should show your attempts at solving the problem before receiving help.

Can you begin to calculate the effect of all your sample dilutions are on your unknown?