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Topic: Method for Fat in Honey  (Read 2376 times)

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Offline ken3009

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Method for Fat in Honey
« on: July 30, 2013, 02:22:08 AM »
i know honey has 0g fat.
what method is used to test it? acid hydrolysis fat (SBR) or dairy fat RG (roese gottlieb) method?
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Offline Archer

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Re: Method for Fat in Honey
« Reply #1 on: August 10, 2013, 01:24:24 AM »
Possibly just something as simple as a petroleum extraction of an aqueous solution of honey.
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Offline gapeev

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Re: Method for Fat in Honey
« Reply #2 on: September 26, 2013, 06:05:29 PM »
Extraction with nonpolar solvent followed by gc-fid or gc-ms: There is very little fat in honey. But I did see triglycerides while looking at pesticides in honey. Let me know if any mor info needed.
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Offline WW

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Re: Method for Fat in Honey
« Reply #3 on: October 18, 2013, 08:54:29 AM »
Bligh/Dyer (1950) extraction.  at least that's the way I would do it.   Then if you want you can saponify the Triacylglycerides and analyze the composition on GC/MS
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