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Topic: "Azeotroping off" Ammonium Bicarbonate  (Read 3518 times)

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Offline wildburg88

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"Azeotroping off" Ammonium Bicarbonate
« on: February 10, 2014, 10:45:01 PM »
I was wondering if someone could fully explain how to "azeotrope off" ammonium bicarbonate.

If I have a peptide solution in (NH4)HCO3 that I then dry down in a SpeedVac / lyophilize, the ammonium, because it is volatile will blow off leaving the bicarbonate in the sample. If I then had 50/50 H2O/MeOH to the completely tried down sample and lyophilize again and repeat this step 1-2 more times, my sample should be almost completely free of any salt.

How exactly does using 50/50 H2O/MeOH get rid of the bicarbonate? The sample is not being heated in the SpeedVac, which is kept at 4°C. Is it something like azeotropic distillation?

Offline Borek

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Re: "Azeotroping off" Ammonium Bicarbonate
« Reply #1 on: February 11, 2014, 03:10:27 AM »
the ammonium, because it is volatile will blow off leaving the bicarbonate in the sample

Try to write reaction equation.
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