As a synthetic guy, I always have to run column. In some cases, unfortunately the polarity of a compound on a column is different suggested from TLC and this is very annoying because the column does not work at all and the Rf of a compound on the column can only be estimated by observing the elution concentration/volume.
I learn from books that this results from the different silica gel size used in the TLC and flash column chromatography.
Have you guys also come across this kind of situation? Any suggestion about how to work with it??