[jiayi0901jia]

hello.
I have a question for this topic. Now I have different size reversed capillary column with dimensions of 15, 50, 75 um ID, ~60 cm and theoretically 1-2 nm coating on the wall. I'm trying to optimize running condition. But I keep getting tailing and broad peaks. I tried flow rate range 0.01 to 0.001 mL/min and injection volume 300, 60 nl, and smaller injection volume by using split injection using T dead connection. I'm trying toluene, naphthalene, phenol for now.
Anyone has any idea on how to optimize it?

[Archer]

What is the stationary phase? What mobile phase are you using?

[jiayi0901jia]

Thanks for replying. it's carbon like structure with aromatic rings, it's theoretically monolayer polymerized structure. I used MeOH : water 3:7, ACN to water 9:1, 4:6, 5:5.