April 29, 2024, 05:22:02 AM
Forum Rules: Read This Before Posting


Topic: spectrophotometric question  (Read 3148 times)

0 Members and 1 Guest are viewing this topic.

akp85

  • Guest
spectrophotometric question
« on: March 21, 2006, 01:09:36 PM »
The procedure calls for analysis at a wavelength where the absorbance is a relative maximum (we recorded our absorbance values at the maximum wavelength for this solution which happened to be 455nm). Suppse there are one or more interfering species in a solution, which also absorb more or less strongly at this same wavelength. How could/should you deal with such a situation of overlapping absorbances?

So yes using spectrophotometric method, how do i avoid overlapping absorbances?


Offline tamim83

  • Retired Staff
  • Full Member
  • *
  • Posts: 395
  • Mole Snacks: +67/-7
  • Gender: Female
  • Quantum Kitten
Re:spectrophotometric question
« Reply #1 on: March 25, 2006, 09:18:40 PM »
You should find the wavelegnth of maximum absorbance for each substance in your solution. You can do this by making solutions of the interfering substances and measuring the absorbance over a vwavelegnth range.  

Sponsored Links