March 29, 2024, 07:24:06 AM
Forum Rules: Read This Before Posting


Topic: Proteins and Uniprot  (Read 1852 times)

0 Members and 1 Guest are viewing this topic.

Offline Lii

  • Regular Member
  • ***
  • Posts: 30
  • Mole Snacks: +1/-0
Proteins and Uniprot
« on: September 20, 2014, 06:38:28 PM »
I'll just say it: proteins REALLY confuse me. UniProt confuses me even more.

Okay, so I have a uniprot number for a specific protein. This protein has an important domain, X. I was supposed to find the most closely related members of the X domain family, so I ran a BLAST on the entire protein and it listed a bunch of other proteins and their percent similarities. So I think I did that right... Or maybe I should just BLAST the domain sequence.

Then I'm asked to produce a sequence alignment and identify the sequence signature for the X domain and I have no idea what to do. I notice there is an align tab but it's not clickable.

After this I have to determine which protein has the shortest X domain using molecular weights. Also not sure if they list molecular weights on there or if I have to calculate them somehow.

If anyone has any tips on how to navigate the site I would be very grateful.

Sponsored Links