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taq thermostable polymerase
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Memy:
Hello,
I have a question on Taq thermostable polymerase.
I have read that this is the most widely used enzyme for PCR tecniques, although it lacks a 3'to5' exonuclease activity.
Thus, why is it that widely used, since the PCR products will have lot of misincorporated nucleotides and thus low fidelity-copying is achieved with the Taq polymerase?
I have also read that it is sometimes selected based upon reliability and fussiness criteria.
Could someone explain what are these referred to?
thank you very much
maria
Equi:
Many PCRs are not done for precise code-copies, but to create band patterns on electrophoresis gels (e.g. DNA fingerprints).
When cloning genes, which need to be expressed later, one usally uses Vent (or Takara Taq) polymerase.
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