Hi there, I was doing HPLC analysis of my reaction and question occured to me:
Is it possible that by changing method - specificaly mobile phase gradient - that peak of one compund change its retention time, while the other doesnt?
compound A: RT 1,04 in one gradient and 1,71 in second
compound B: RT 1,33 in one gradient and 1,31 in second
Ive seen that usualy both compound change their retention times but Ive never seen that only one does. I see no reason why this couldnt happen but it seems weird.