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Topic: Conversion of Ethanol to Acetaldehyde with H202 and Catalase  (Read 2327 times)

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Offline WTK28

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Conversion of Ethanol to Acetaldehyde with H202 and Catalase
« on: August 18, 2015, 11:49:29 AM »
So I am trying to determine how much H202 and Catalase I need to combine with ethanol to completely convert all the ethanol to Acetaldehyde without leaving excess H202 and Catalase unreacted. Any ideas?

Offline Babcock_Hall

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Re: Conversion of Ethanol to Acetaldehyde with H202 and Catalase
« Reply #1 on: August 18, 2015, 12:13:33 PM »
Is catalase a reactant or a catalyst?  My first thought is to ask whether or not you are sure that ethanol is a substrate for catalase.  My second thought is to suggest that you look into alcohol oxidase.

Offline WTK28

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Re: Conversion of Ethanol to Acetaldehyde with H202 and Catalase
« Reply #2 on: August 18, 2015, 01:43:12 PM »
In short.  Catalyst.

I am trying to understand how ethanol is converted to acetaldehyde in the peroxisomes of the cell using catalase protein and H2O2, and then replicate that experiment in vitro. 

Offline Babcock_Hall

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Re: Conversion of Ethanol to Acetaldehyde with H202 and Catalase
« Reply #3 on: August 18, 2015, 02:15:03 PM »
Catalase converts hydrogen peroxide into water and oxygen.  I suspect that a different enzyme is responsible for oxidizing ethanol, but I don't have detailed knowledge of the peroxisomes.

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