[AmberWoo]

I have been looking for what exactly happens, what parts are turning on and moving and how does it know what to adjust, but I haven't  been able to find an answer so I am hoping someone here can help me.  All I've been able to find is the information that is given during an Autotune not how it obtained that information.

Thanks so much.

[Arkcon]

Here's a useful article:  http://www.sepscience.com/Techniques/LC/Articles/695-/HPLC-Solutions-5-LCMS-Calibration-vs-Tuning  What are you actually doing, calibration or tuning, and why are you doing it?