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Topic: Yellow Colored Solution in Acid Digestion  (Read 2757 times)

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Offline stdacet

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Yellow Colored Solution in Acid Digestion
« on: December 06, 2015, 02:21:22 AM »
For wet acid digestion from fish meat matrix, currently I use conc. HNO3, hydrogen peroxide (1:1) (10 ml). The solution that remains after digestion should be colorless, but I always get solution with yellow color. After evaporation, the volume of the solution remains about 5 ml, but still its yellow.

According to literature,  the hydrogen oxide suppresses the yellow nitrous oxides, but still I am getting it.

Can anyone suggest a solution for that.
Thanks

Offline discodermolide

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Re: Yellow Colored Solution in Acid Digestion
« Reply #1 on: December 06, 2015, 09:58:53 AM »
What concentration of hydrogen peroxide are you using?
Maybe you need to ensure it is still OK?
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Offline stdacet

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Re: Yellow Colored Solution in Acid Digestion
« Reply #2 on: December 07, 2015, 12:05:54 AM »
Hydrogen peroxide is about 34.5 %, stored properly in dark.

Offline discodermolide

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Re: Yellow Colored Solution in Acid Digestion
« Reply #3 on: December 07, 2015, 04:14:09 AM »
It is still worth making sure it is still 34.5%. Especially if it has been used several times.
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Offline stdacet

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Re: Yellow Colored Solution in Acid Digestion
« Reply #4 on: December 07, 2015, 12:58:39 PM »
Ok, I'll check it.  Can the amount of nitric acid added be a limiting factor, ? say for 10 g sample, 5 ml conc. nitric acid may not be enough?

Offline discodermolide

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Re: Yellow Colored Solution in Acid Digestion
« Reply #5 on: December 07, 2015, 01:09:05 PM »
No idea, but probably not. I would go for the peroxide. But check the nitric as well.
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