[wm334]

Hi,

I want to measure a hydrophobically modified polymer via fluoresence to see how stable it is in varying concentrations.

I have read in journals to put the probe in water at 1e-6 M but im told this will cause too many interactions in spectroscopy and dilution is too great.

What should I be putting the probe in for use with the polymer?

Regards

[Corribus]

This post is very vague. Let's start with: How do you intend to use fluorescence to measure stability, and what probe are you intending to use? I'm also not sure what your second sentence really means. Whether or not a probe is compatible with water depends largely on what it is and what you're trying to measure with it.

[wm334]

I want to look at how well the pyrene hydrophibic probe sits in my hydrophobic polymer at different concentrations

The probe is 1-3bis pyrene and is solid

[Corribus]

At different concentrations of what - polymer or probe?

Is your hypothesis that the probe will be buried in the hydrophobic regions of the coiled polymer? Is this method published elsewhere?

[wm334]

Sorry at different concentrations of polymer

My colleague has previously used this method to study the probe in the hydrophobic but unfortunately she is away