September 18, 2021, 07:07:05 AM
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Topic: review article on storage conditions for enzymes  (Read 2614 times)

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Offline Babcock_Hall

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review article on storage conditions for enzymes
« on: June 02, 2016, 09:43:44 AM »
Many years ago I found a lengthy review article that was a survey of how various enzymes were stored.  It had conditions under which enzymes were frozen and perhaps lyophilized IIRC.  Recently I went through my massive collection of photocopied articles but I could not find it.  I seem to recall that there were two authors, but I do not remember the name of the journal.  The article was probably written in the 1990s, but I could be off by a few years on either side.   I have tried extensive searches using Pubmed and less extensive searches using Google Scholar, but they have not produced the article I have in mind.  Does it ring any bells with anyone?  Any suggestions for the best way to search for it?

Offline Yggdrasil

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Re: review article on storage conditions for enzymes
« Reply #1 on: June 02, 2016, 11:14:29 AM »
Not sure about finding your specific article, but google gives a few good guides about conditions for protein storage:
https://www.embl.de/pepcore/pepcore_services/protein_purification/storage_purified_proteins/
http://onlinelibrary.wiley.com/doi/10.1002/0471140864.ps0406s27/pdf
http://www.indiana.edu/~lchenlab/protocol_files/protein_storage.pdf

Maybe try looking in one of the Current Protocols, CSH protocols, Methods in Enzymology, or other methods focused journal.

Offline Babcock_Hall

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Re: review article on storage conditions for enzymes
« Reply #2 on: July 06, 2021, 06:06:24 PM »
Enzyme stabilization: state of the art
Liliana Gianfreda & Maria Rosaria Scarfi
Molecular and Cellular Biochemistry volume 100, pages 97–128 (1991)
https://doi.org/10.1007/BF00234161

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