Hi, I have two questions that interest me related of proteins and PI:
1) Phosphorylation is a modification after the translation of of proteins. The addition of molecular mass protein X after phosphorylation is 80Da, when only a part (!) Of the molecules of the protein takes to phosphorylation.
Is it true that you can identify phosphorylation through differences in pI of the protein phosphorylated and non-phosphorylated?
2) Is it correct to say of proteins may pass a greater distance in SDS PAGE If the PI are lower, even that the Mw (Dalton kilobytes) bigger?
Thank you very very much!