March 28, 2024, 03:56:24 PM
Forum Rules: Read This Before Posting


Topic: HPLC mobile phase question  (Read 2108 times)

0 Members and 1 Guest are viewing this topic.

Offline jvm208

  • Regular Member
  • ***
  • Posts: 17
  • Mole Snacks: +0/-0
HPLC mobile phase question
« on: January 14, 2017, 01:42:39 AM »
Could someone please explain why retention would increase as the acetonitrile
portion of the mobile phase increases in HPLC?

Would this apply to non-polar analytes?

Thank you.

Offline Dan

  • Retired Staff
  • Sr. Member
  • *
  • Posts: 4716
  • Mole Snacks: +469/-72
  • Gender: Male
  • Organic Chemist
    • My research
Re: HPLC mobile phase question
« Reply #1 on: January 14, 2017, 07:38:21 AM »
Could someone please explain why retention would increase as the acetonitrile
portion of the mobile phase increases in HPLC?

Depends on whether the the stationary phase is normal or reverse phase (RP), and it depends on the composition of the mobile phase (what is the other solvent?).
My research: Google Scholar and Researchgate

Offline jvm208

  • Regular Member
  • ***
  • Posts: 17
  • Mole Snacks: +0/-0
Re: HPLC mobile phase question
« Reply #2 on: January 15, 2017, 01:31:13 AM »
The mobile phase composition is acetonitrile and water (75:25) in reversed phase HPLC

Thank you.

Sponsored Links