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Topic: HPLC mobile phase question  (Read 1600 times)

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Offline jvm208

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HPLC mobile phase question
« on: January 14, 2017, 01:42:39 AM »
Could someone please explain why retention would increase as the acetonitrile
portion of the mobile phase increases in HPLC?

Would this apply to non-polar analytes?

Thank you.

Offline Dan

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Re: HPLC mobile phase question
« Reply #1 on: January 14, 2017, 07:38:21 AM »
Could someone please explain why retention would increase as the acetonitrile
portion of the mobile phase increases in HPLC?

Depends on whether the the stationary phase is normal or reverse phase (RP), and it depends on the composition of the mobile phase (what is the other solvent?).
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Offline jvm208

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Re: HPLC mobile phase question
« Reply #2 on: January 15, 2017, 01:31:13 AM »
The mobile phase composition is acetonitrile and water (75:25) in reversed phase HPLC

Thank you.

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