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Topic: azo-dye spectrophotometry color development fails  (Read 1182 times)

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Offline vsagikis

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azo-dye spectrophotometry color development fails
« on: January 20, 2017, 02:46:06 AM »
Why this reaction occasionally produce conglomerates instead of the nice purple color we use to quantify parabenzoic acid?

Reaction
1. Sample containing parabenzoic acid as analyte + NaOH; boiling; + HCl to pH 2
2. + sodium nitrate - to form the azo bonds
3. + sulfanilamide - to react extra nitrite
4. + azo-dye: (N-(1-Naphthyl)ethylenediamine dihydrochloride (NED))



the more analyte in the sample the faster the sediment shows up in the sample, the color is pinkish when sediments present

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