I am having trouble with solid phase extraction and was wondering if anyone could help.
All I seem to get so far, is that SPE is empiric (translate that as - trial and error)
I'm not too keen on trying out millions of possibilities and wasting so many expensive cartridges.
anyway, I have an extraction mixture (which should include my organophosphorus pesticides) in cyclohexane, and would like to clean it up through an SPE cartidridge, now usually C18 cartidges are used, but C18 seems to be used with methanol or acetonitrile.
another paper has used florisil cartridges, but i've heard elsewhere that there is analyte loss with florisil.
so could i put cyclohexane through a C18, and would it do anything?
if i really must use cyclohexane, how should i prepare the cartridge?
what would be a good eluant?
is this the right place to post this kind of question anyway?
thanks in advance