I have to make a Resuspension buffer for a plasmid exaction, The buffer is based on Tris Base and EDTA, with RNAse A added.
The components are:
-50mM Tris-HCl (pH8)
-10mM EDTA (im using EDTA disodium dihydrate)
-100 microgram/mL RNAse A
I don't have stock solutions, all I have are the salts for each compound.
My plan was to first make a 50mL solution of 50mM Tris, then pH it to 8 with HCl.
Then make a 50mL 10mM EDTA solution, and add it to the Tris.
Then just add the RNAse A after.
But then I realized I would just diluting everything if I do it this way.
I really don't know how to start.