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Topic: How to prepare a 1M Citrate-Phosphate Buffer pH 7.4 ?  (Read 1452 times)

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Offline Ploaka75

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How to prepare a 1M Citrate-Phosphate Buffer pH 7.4 ?
« on: October 09, 2018, 02:35:09 PM »
Hi Folks,

for work I have to establish a recipe for preparation of a 1 M citrate-phosphate buffer pH 7.4, which should be composed of Na2HPO4 and citric acid. Both ingredients should be at sufficiently high concentrations to allow for preparation of the 1M solution at a final volume of approx. 50 to 200 mL. However, I am unfortunately not familiar with buffer calculations for chemistry. Also, on the web I have only found recipes for diluted versions of this buffer, that is also known as McIlvaine buffer and I am aware that there exist other buffer systems which are more suitable for buffering at a pH of 7.4. Please can you help me and propose me a recipe for a 1 M McIlvaine buffer pH 7.4 ? Many thanks in advance!
Kind regards,

Ploaka  :)
« Last Edit: October 09, 2018, 03:07:00 PM by Ploaka75 »

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