Hi all, I'm working on developing a nanoparticle formulation where two components, one in water (mRNA) and one in ethanol (lipid) is mixed. I need to run it through a TFU but before I do I need to reduce the volume of ethanol to below 17%. Does anyone have any ideas of how to do this without breaking my nanoparticles? i.e. gently, without boiling, etc.
At the moment I've been trying dilutions, blending then doing two separate dilutions. This reduces the ethanol concentration enough but Im left with large volumes and low particle concentration.
My background is in chemistry so not used to being so careful with biological material.