July 07, 2020, 02:57:28 PM
Forum Rules: Read This Before Posting

Topic: Chromatography used in medicinal plant science  (Read 1576 times)

0 Members and 1 Guest are viewing this topic.

Offline Chantelle C.

  • Very New Member
  • *
  • Posts: 1
  • Mole Snacks: +0/-0
Chromatography used in medicinal plant science
« on: November 13, 2018, 02:07:42 AM »
Hi, in our one exam example we have a question regarding which chromatography procedure will be used for a large sample size as in 400g or more no other information is given please help.

Offline jeffmoonchop

  • Full Member
  • ****
  • Posts: 212
  • Mole Snacks: +19/-2
  • Gender: Male
Re: Chromatography used in medicinal plant science
« Reply #1 on: November 13, 2018, 12:33:45 PM »
Assuming the sample is homogeneous, you dont need to analyse the whole sample. Just taking a few small samples from the batch will give you confirmation it is what it is. Run all the usual analyses on 6 smaller samples taken after mixing the large sample to ensure precision. I dont know of anyone who would run 400g sample in a chromatography method. If you have to use it all, dissolving it in a large flask would give you true values, but again you would only use around 1ml in a HPLC vial.

Sponsored Links