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Author Topic: pH calculation help  (Read 673 times)

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pH calculation help
« on: December 06, 2018, 01:52:12 PM »

We did an experiment to estimate the isoelectric point of Casein by pH/Turbidity Titration.

In the practical we made up solutions of 9ml using different volumes of acid and distilled water. Here are 3 examples; it's been a long time since I've done this and would really appreciate help with the method.
                              A         B           C
Distilled Water      8.40      7.75      6.50
0.01M Acetic acid  0.60      1.25      2.50

PKa = 4.76

My initial reaction was to get the Ka via the Pka and then using that to get the [H+] that can then be used to get the pH but that method would give me the same answer for all of them? Any help would be greatly appreciated.


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Re: pH calculation help
« Reply #1 on: December 06, 2018, 09:05:08 PM »

Each prepared sample has a different concentration of acetic acid.
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation,,, PZWT_s1

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