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Author Topic: Question about type of acid used in Riboflavin assay  (Read 685 times)

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Sgriger534

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Question about type of acid used in Riboflavin assay
« on: December 17, 2018, 02:36:08 PM »

So I am currently working on updating some analytical procedures where I work and one is a fluorometric riboflavin assay

My question is that the procedure uses 0.2n Hcl to dissolve the sample.  However every other similar procedure I can find (e.g. USP, AOAC) uses 0.2n acetic acid.
Is there any benefit to using one acid over the other?
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Babcock_Hall

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Re: Question about type of acid used in Riboflavin assay
« Reply #1 on: December 17, 2018, 04:13:13 PM »

I don't know the answer, but there is going to be a large pH difference, and I would at least consider how stable riboflavin is at the lower pH value.
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Sgriger534

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Re: Question about type of acid used in Riboflavin assay
« Reply #2 on: December 17, 2018, 04:22:25 PM »

As in the other methods,  the ph is adjusted (although I can't remember the value currently) but it falls within the same range as the methods using acetic acid
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