I'm currently doing some tests on the robustness of a liquid-liquid extraction and i have some results i cannot explain and cannot find in literature.
When i repeat the extraction multiple times, al my components mostly stay within 10% around the mean extraction yield. But some components (palmitic acid, erucamide and n-heptacosane) vary a lot and non consistent between tests. Sometimes only palmitic acid varies, sometimes all three.
I have attached a screenshot of one of my tests with most components censored because of secrecy reasons.
Thanks in advance!
All tests are spiked with an automatic syringe from the same stock solution.
Every extraction test consist of 3 extractions at varying pH (Neutral, acidic, alkaline)
Extracion yield is the area under a peak on a GCMS measurement.