Hi all, this is my first post
I need to make isotopically enriched phosphoserine, but I am not so great with organic chemistry. Although I can buy phosphoserine from any supplier, nobody sells it isotopically enriched so I must make it myself.
I am having a hard time planning out what to do because there are 3 functional groups on serine, but I want to selectively phosphorylate the serine side chain hydroxyl. In general, my current plan is the following:
1: N-boc protect L-serine
2: C-protect boc-L-serine with
(I need help here, I want something that cleaves with TFA)
3: phosphorylate free hydroxyl using a phosphoramidite
4: oxidize phosphate with tBuOOH
5: cleave all protecting groups with TFA
Does anybody see any flaws with this general plan, or see any easier options? Am I missing anything that would allow me to selectively phosphorylate the serine hydroxyl?
thank you
-pigsknow