:)hi ,ı want to learn analys following;

.. stirrer. At end of 20min, transfer 50mL of liquid to centrifuge tube. Then centrifuge at 6000rpm . 5min. Collect supernatant for Kjeldahl method. And now here is my problem. How many . should I use as sample in Kjeldahl? And how to calculate for the protein solubility? . understand. Example: Sample weight: 1.5072g Liquid Kjeldahl value: 1.125 mg N/ml Dry Kjeldahl value: . .47% 1.125 mg N/mL x 75mL (how much KOH added) = 84.4mg N / . .5072g = 55.98mg N x 100g sample = 5.598g N 5.598g N * 6.25 = 34. . (34.99/47.47) x 100 = 73.68% solubility Why 84.4mg N need to divide by sample weight? And why 55.98 ..