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Topic: about KOH Protein Solubility  (Read 1736 times)

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Offline semac

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about KOH Protein Solubility
« on: February 15, 2016, 04:04:04 AM »
 :)hi ,ı want to learn analys following;

..  stirrer. At end of 20min, transfer 50mL of liquid to centrifuge tube. Then centrifuge at 6000rpm  .  5min. Collect supernatant for Kjeldahl method.  And now here is my problem. How many  .  should I use as sample in Kjeldahl? And how to calculate for the protein solubility?  .  understand.   Example: Sample weight: 1.5072g Liquid Kjeldahl value: 1.125 mg N/ml Dry Kjeldahl value: . .47%  1.125 mg N/mL x 75mL (how much KOH added) = 84.4mg N /  . .5072g = 55.98mg N x 100g sample = 5.598g N 5.598g N * 6.25 = 34. .  (34.99/47.47) x 100 = 73.68% solubility   Why 84.4mg N need to divide by sample weight? And why 55.98  ..

Offline Borek

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Re: about KOH Protein Solubility
« Reply #1 on: February 15, 2016, 05:17:27 AM »
This is barely readable. If you want someone to help, please post your question in a way that is easy to comprehend.
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