I understand that the beer lamert law states: A=ecl, and that the spectrophotometer measures the transmitted light and so therefore using a lower concentration would give a larger transmission of light and give lower error.
How do you find the optimum dilution of a stock solution in order to give an absorbance of low error?
Q. by what factor would you dilute 1mmol/dm^3 solution of NADH (e= 6.22 x 10^6 cm^2mol-1) to measure its absorbance accurately.
Q. how can the beer lambert law be used to determine the optimum condition for the most accurate absorbance